4,007 research outputs found
Project- and Group-Based Learning of Junior Writing in Biology
Writing in Biology, part of the Junior Writing Program, is inherently a project-based learning course. After a Science, Technology, Engineering, and Mathematics Teacher Education Collaborative (STEMTEC) workshop, the course was thoroughly revised. Each of six projects was modified to increase student-active and group participation. Base groups with a balanced experience constitution are established using voluntary ordering and random assignment. A walk-around during the initial meeting serves to establish bonding within the base groups. Random groups are used within exercises to stimulate student interaction and familiarity with ad hoc group cooperation. Digital images of, and by, students are used to encourage student interaction and name recognition. A website with the entire course plan is available at an archival site to complement and help elucidate the course
CâC chemokineâinduced eosinophil chemotaxis during allergic airway inflammation
The production of eosinophilâspecific chemotactic factors during allergic airway responses may be a pivotal event resulting in eosinophil accumulation, activation, and airway damage. Recent studies have identified specific chemokines that may play crucial roles in recruitment of eosinophils to the site of allergic reactions. In this study we have utilized an established model of schistosome egg antigen (SEA)âmediated allergic responses to examine the role of specific CâC chemokines [macrophage inflammatory proteinâ1α (MIPâ1α), RANTES, and monocyte chemoattractant proteinâ1 (MCPâ1)] in eosinophil recruitment. We have previously identified a role for MIPâ1α in eosinophil accumulation in the lung and airway during allergic airway inflammation. We extend those studies using in vitro eosinophil chemotaxis to establish that both MIPâ1α and RANTES are potent eosinophil chemotactic factors in lungs during allergic airway responses. Morphometric analysis demonstrated a peribronchial accumulation of eosinophils within the lungs beginning at 8 h, peaking at 24 h, and plateauing at 48â96 h after allergen (SEA) challenge. Utilizing wholeâlung homogenates from allergenâchallenged mice, in vitro eosinophil chemotactic assays demonstrated significant increases in eosinophil chemotactic activity with 8âh lung homogenates and peak activity with samples from 24âh lung homogenates. These data correlated with the morphometric analysis of peribronchial eosinophil accumulation in situ. When lung homogenates from allergenâchallenged mice were preincubated in vitro with antibodies specific for MIPâ1α, RANTES, or MCPâ1, a significant reduction in eosinophil chemotaxis was observed with only MIPâ1α and RANTES neutralization. Altogether, these studies indicate that RANTES and MIPâ1α are major eosinophil chemotactic factors produced during allergic airway responses. J. Leukoc. Biol. 60:573â578; 1996.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141543/1/jlb0573.pd
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